Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemPhosphatase Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5870S
Quantity1 ml
Research AreaRelated Products
ApplicationsW
Clone100X

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stressbioRxivDecember 1, 2023Manuel A. Torres Acosta, Nurbek Mambetsariev, Carla P Reyes Reyes Flores, Kathryn A. Helmin, Qianli Liu, Anthony M. Joudi, Luisa Morales-Nebreda, Jonathan Gurkan, Kathleen Cheng, Hiam Abdala-Valencia, et al.lysis buffer (Cell Signaling cat. no. 9803) supplemented with phosphatase (Cell Signaling cat. no. 5870S) and protease inhibitors (Roche, cat. no. 65726900) after which their concentration was measured with

(2) Scaffold-induced compression enhances ligamentization potential of decellularized tendon graft reseeded with ACL-derived cellsiScienceNovember 23, 2023Jinsung Park, Hyunsoo Soh, Sungsin Jo, Subin Weon, Seung Hoon Lee, Jeong-Ah Park, Myung-Kyu Lee, Tae-Hwan Kim, Il-Hoon Sung, Jin Kyu Leefollowing the conventional methods with 1x RIPA buffer containing phosphatase inhibitor (Cell Signaling, 5870S) and protease inhibitor (Calbiochem, 535140) on ACL-derived cells. Quantification of extracted proteins

(3) Adrenomedullin has a pivotal role in trophoblast differentiation: A promising nanotechnology-based therapeutic target for early-onset preeclampsiaScience AdvancesNovember 3, 2023Qingqing Zhang, Cheuk-Lun Lee, Tingyu Yang, Jianlin Li, Qunxiong Zeng, Xiaofeng Liu, Zhongzhen Liu, Degong Ruan, Zhuoxuan Li, Anita S. Y. Kan, et al.containing 1% proteinase inhibitor cocktail (11836170001, Roche) and 1% phosphatase inhibitor cocktail (5870, Cell Signaling Technology) followed by centrifugation at 12,000 rpm and 4°C for 30 min. Protein concentrations

(4) Elevated BMPR2 expression amplifies osteoblast differentiation in ankylosing spondylitisJournal of Rheumatic DiseasesOctober 1, 2023Sungsin Jo, Seung Hoon Lee, Chanhyeok Jeon, Hye-Ryeong Jo, Eunae Ko, Min Whangbo, Tae-Jong Kim, Ye-Soo Park, Tae-Hwan KimThermo Fisher Scientific). The cells were lysed with 1× radioimmunoprecipitation assay buffer containing phosphatase (5870S; Cell Signaling Technology, Danvers, MA, USA) and protease (#535140; Calbiochem, San Diego, CA, USA)

(5) Selective Activation of M1 Muscarinic Receptors Attenuates Human Colon Cancer Cell ProliferationCancers (Basel)September 28, 2023Margaret H. Sundel, Natalia Sampaio Moura, Kunrong Cheng, Oscar Chatain, Shien Hu, Cinthia B. Drachenberg, Guofeng Xie, Jean-Pierre Raufmancontaining Cell Lysis Buffer (catalog #9803), anti-protease (catalog #5871), and anti-phosphatase (catalog #5870)—all from Cell Signaling. Cell lysates were centrifuged at 15,000× g at 4 °C for 10 min. Supernatants

(6) HER2-Selective and Reversible Tyrosine Kinase Inhibitor Tucatinib Potentiates the Activity of T-DM1 in Preclinical Models of HER2-positive Breast CancerCancer Research CommunicationsSeptember 25, 2023Devra Olson, Janelle Taylor, Kelsi Willis, Kelly Hensley, Sean Allred, Margo Zaval, Lauren Farr, Robert Thurman, Nishi Jain, Renee Hein, et al.Protease Inhibitor Cocktail (Millipore Sigma; #535140) and 1:100 Phosphatase Inhibitor Cocktail (CST; #5870S). Protein concentration of lysates was measured using a bicinchoninic acid assay. Protein levels were

(7) NRP1 transduces mechanical stress inhibition via LATS1/YAP in hypertrophic scarsCell Death DiscoverySeptember 13, 2023Mengzhi Li, Peng Wang, Jingting Li, Fei Zhou, Shixin Huang, Shaohai Qi, Bin ShuUSA) containing a protease inhibitor cocktail and phosphatase inhibitor cocktail (1:100; #5871 and #5870, respectively; Cell Signaling Technology, Danvers, MA, USA). Briefly, 20 µl of each sample was loaded

(8) Δ9-Tetrahydrocannabinol does not upregulate an aversive dopamine receptor mechanism in adolescent brain unlike in adultsCurrent Research in NeurobiologySeptember 4, 2023Marie-Eve Di Raddo, Marija Milenkovic, Meenalochani Sivasubramanian, Ahmed Hasbi, Jack Bergman, Sarah Withey, Bertha K. Madras, Susan R. Georgecontaining protease inhibitor (Sigma, P8340) and phosphatase inhibitor (Cell Signaling Technology, 5870 S). Samples were maintained in constant agitation for 30 min then centrifuged at 10 000 g for 10 min

(9) Inhibition of DNMT1 methyltransferase activity via glucose-regulated O-GlcNAcylation alters the epigenomeeLifeJuly 20, 2023Shin H, Leung A, Costello KR, Senapati P, Kato H, Moore RE, Lee M, Lin D, Tang X, Pirrotte P, et al.presence of a protease inhibitor (Cat# 8340; Sigma-Aldrich) and a phosphatase inhibitor cocktail (Cat# 5870; Cell Signaling) for the western blotting or immunoprecipitation. An increase in fasting blood glucose

(10) Mechanism and therapeutic implications of pomalidomide-induced immune surface marker upregulation in EBV-positive lymphomasScientific reportsJuly 18, 2023Jaeger HK, Davis DA, Nair A, Shrestha P, Stream A, Yaparla A, Yarchoan R.phosphorylated AKT were extracted in the presence of both phosphatase inhibitor cocktail (Cell signaling cat#5870) and protease inhibitor cocktail (ThermoFisher Scientific) at a final 1X concentration. Protein concentrations

(11) SMYD5 is a novel epigenetic gatekeeper of the mild hypothermia responsebioRxiv : the preprint server for biologyJune 9, 2023Rafnsdottir S, Jang K, Halldorsdottir ST, Tomasdottir A, Vinod M, Möller K, Reynisdottir T, Atladottir LH, Allison KE, He J, et al.1% NP-40, 1% Sodium deoxycholate, 0.1% SDS, 2mM EDTA, phosphatase inhibitor (either Cell signaling, 5870S or Thermo Fisher Scientific, 78437)). The lysate was centrifuged at 16,000 ×g for 20 min at 4°C and

(12) Hyponatremia-related liver steatofibrosis and impaired spermatogenesis: evidence from a mouse model of the syndrome of inappropriate antidiuresisJournal of endocrinological investigationMay 1, 2023Marroncini G, Anceschi C, Naldi L, Fibbi B, Brogi M, Lanzilao L, Fanelli A, Maggi M, Peri A.supplemented with protease (11,697,498,001, Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (#5870, Cell Signaling Technology, Danvers, Massachusetts, USA) 100X, to obtain whole protein lysate for immunoblot

(13) Phosphatase regulatory subunit MYPT2 knockout partially compensates for the cardiac dysfunction in mice caused by lack of myosin light chain kinase 3Journal of Biological ChemistryApril 1, 2023Tingfei Hu, Hema Kalyanaraman, Renate B. Pilz, Darren E. CasteelCorporation (Cat#: 539131) and phosphatase inhibitor cocktail was from Cell Signaling Technology (Cat#: 5870). Horseradish peroxidase (HRP)-conjugated anti-Flag antibody was from Sigma-Aldrich (Cat#: A8592). Rabbit

(14) Activation of the PI3K/AKT/mTOR Pathway in Cajal–Retzius Cells Leads to Their Survival and Increases Susceptibility to Kainate-Induced SeizuresInternational Journal of Molecular SciencesMarch 11, 2023Nasim Ramezanidoraki, Driss El Ouardi, Margaux Le, Stéphanie Moriceau, Mahboubeh Ahmadi, Elena Dossi, Danae Rolland, Philippe Bun, Gwenaëlle Le Pen, Guillaume Canaud, et al.(Saint-Louis, MI, USA) with protease (ref: #04693116001; Roche (Bâle, Switzerland) and phosphatase (ref: #5870; Cell Signaling (Danvers, MA, USA)) inhibitors. Following this, 25 µg of the total protein extracts

(15) Extracellular PPM1A promotes mineralization of osteoblasts differentiation in ankylosing spondylitis via the FOXO1A‐RUNX2 pathwayJournal of Cellular and Molecular MedicineMarch 1, 2023Subin Weon, Sungsin Jo, Bora Nam, Sung Hoon Choi, Ye‐Soo Park, Yong‐Gil Kim, Tae‐Hwan KimX‐100) containing protease inhibitors (Calbiochem, 535140) and phosphatase inhibitors (Cell Signalling, 5870 S). Lysed samples were incubated on ice for 15 min followed by centrifugation at 12,000× g for 15 min

(16) New Glucosamine-Based TLR4 Agonists: Design, Synthesis, Mechanism of Action, and In Vivo Activity as Vaccine AdjuvantsJournal of Medicinal ChemistryFebruary 23, 2023Alessio Romerio, Nicole Gotri, Ana Rita Franco, Valentina Artusa, Mohammed Monsoor Shaik, Samuel T. Pasco, Unai Atxabal, Alejandra Matamoros-Recio, Marina Mínguez-Toral, Juan Diego Zalamea, et al.(CST, #9806), supplemented with protease (Roche, Mannheim, Germany) and phosphatase inhibitors (CST ##5870). After centrifugation at 13,000 RCF for 30 min at 4 °C, the supernatants were collected as whole-cell

(17) TRPC7 facilitates cell growth and migration by regulating intracellular Ca2+ mobilization in lung adenocarcinoma cellsOncology LettersJanuary 24, 2023Jui-Lin Liang, Ming-Hsien Tsai, Yi-Chun Hsieh, Huei-Syuan Liu, Shao-Wei Chen, Yung-Yun Huang, Li-Ching Lin, Tsung-Fu Tsai, Yun-Fang Liang, Wen-Li Hsucontaining proteinase (cat. no. S8830-20TAB; Sigma-Aldrich; Merck KGaA) and phosphatase inhibitors (cat. no. 5870S; Cell Signaling Technology, Inc.). Cell debris were removed by centrifugation at 14,000 × g for 30 min

(18) Torin1 restores proliferation rate in Charcot-Marie-Tooth disease type 2A cells harbouring MFN2 (mitofusin 2) mutationActa MyolDecember 31, 2022Paola Zanfardino, Alessandro Amati, Easter Anna Petracca, Filippo M. Santorelli, Vittoria PetruzzellaScientific, 89900) supplemented with proteases (Cell Signaling, 5871) and phosphatase (Cell Signaling, 5870S) inhibitors. The cells were sonicated on ice and centrifuged for 10 min at 16,000×g at 4°C and the protein

(19) 1α, 25-Dihydroxyvitamin D3 protects gastric mucosa epithelial cells against Helicobacter pylori-infected apoptosis through a vitamin D receptor-dependent c-Raf/MEK/ERK pathwayPharmaceutical BiologyDecember 31, 2022Shuai Zhao, Daihong Wan, Yaoyao Zhong, Xiwei XuUSA). RIPA buffer (#9806), protease inhibitor cocktail (#7012) and phosphatase inhibitor cocktail (#5870) were purchased from Cell Signalling Technology. Cell and bacterial culture Human gastric mucosa epithelial cell

(20) CYFIP1 Dosages Exhibit Divergent Behavioral Impact via Diametric Regulation of NMDA Receptor Complex Translation in Mouse Models of Psychiatric DisordersBiological PsychiatryNovember 1, 2022Nam-Shik Kim, Francisca Rojas Ringeling, Ying Zhou, Ha Nam Nguyen, Stephanie J. Temme, Yu-Ting Lin, Stephen Eacker, Valina L. Dawson, Ted M. Dawson, Bo Xiao, et al.cocktail,Roche,11873580001, Chemical Compound or Drug,Phosphatase Inhibitor Cocktail,Cell Signaling,5870, Chemical Compound or Drug,Protease Inhibitor Cocktail,Sigma,P8340, Chemical Compound or Drug,4x Laemmli Sample Buffer

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Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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